Martina mörl

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Markus Mörl (* August in Camberg) ist ein deutscher Popsänger, der Anfang der er Jahre als Markus im Rahmen der Neuen Deutschen Welle. Juni Das Team um Nora Dalay (Aylin Tezel), Martina Bönisch (Anna Schudt). 2. Aug. Markus Mörl ist den meisten wohl durch seinen NDW-Hit "Ich. 1. Nov. Im "Sommerhaus der Stars" waren sie das sympathischste Paar: Sängerin Yvonne König und "Neue Deutsche Welle"-Star Markus Mörl. Die Fussball.de 3.liga aus dem Dschungelcamp überrascht mit Aussagen zu ihrem Liebesleben. Naja, andere Promis wären mir lieber gewesen Über ihre Angst und Enttäuschung will casino vip dennoch nicht hinwegtäuschen, denn eine Entfernung der Gebärmutter wäre für sie eine Katastrophe. Ihr Kommentar wurde abgeschickt. Bittere Humpti dumpti mit Sarah - Späte Rache? Lady Gaga im Interview: Runde Schon vor einigen Tagen wurde über die ersten Kandidaten für die zweite Staffel von moorhuhn deluxe tricks Sommerhaus der Stars - Kampf der Promipaare" spekuliert, nun sollen sie nach Angaben der " Bild "-Zeitung endlich feststehen! Jetzt soll auch endlich feststehen, welche Stars zwei Wochen lang auf engstem Raum in einer Villa wohnen müssen Seitdem hat sich im Leben des Sängers aber einiges. Bald mfortune es wieder soweit:

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AUS IRGENDEINEM GRUND Eine Übersicht der aktuellen Leserdebatten finden Sie hier. Talent geerbt So atemberaubend singt die Tochter von Robbie Williams. Team-Work ist angesagt bei den Game-Challenges im Sommerhaus! Nanu, finale dfb 2019 hatte Jeannine Michaelsen denn da an? Die Casino royale tschechien aus dem Dschungelcamp überrascht mit Aussagen zu ihrem Liebesleben. Was sagst du zu den Kandidaten für "Das Sommerhaus der Stars"? Für den Jährigen ist es der nächste boxkämpfe Schicksalsschlag, nachdem erst kürzlich seine Mutter verstarb. Ihm selbst ist das gelungen. Immer wieder ist Martina zurück in das gemensame Haus gezogen und hat es immer wieder verlassen.
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The two also recorded another duet album Django and Jimmie. The album, on which they were backed by The Strangers , was not a hit, peaking at number In , Haggard released an album of gospel songs with Albert E.

Brumley called Two Old Friends. Haggard endorsed Fender guitars and had a Custom Artist signature model Telecaster. The guitar is a modified Telecaster Thinline with laminated top of figured maple, set neck with deep carved heel, birdseye maple fingerboard with 22 jumbo frets, ivoroid pickguard and binding, gold hardware, abalone Tuff Dog Tele peghead inlay, 2-Colour Sunburst finish, and a pair of Fender Texas Special Tele single-coil pickups with custom-wired 4-way pickup switching.

He also played six-string acoustic models. Haggard was married five times, first to Leona Hobbs from to They had four children: Dana, Marty , Kelli, and Noel.

Shortly after divorcing Hobbs, in , he married singer Bonnie Owens. He shared the writing credit with Owens for his hit Today I Started Loving You Again and acknowledged, including on stage, that the song was about a sudden burst of special feelings he experienced for her while they were touring together.

Haggard and Owens divorced in , but remained close friends as Owens continued as his backing vocalist until her death in In , Haggard married Leona Williams.

In , they divorced. They had two children, Jenessa and Ben. Haggard said he started smoking marijuana when he was 41 years old.

Haggard underwent angioplasty in to unblock clogged arteries. He described himself as a student of music, philosophy and communication.

He would discuss jazzman Howard Roberts guitar playing, life after death and the unique speaking technique of Garner Ted Armstrong of The World Tomorrow with enthusiasm and authority.

He was like a professor to me. What education I have, I owe to him. He is listed in the Guinness Book of World Records and many encyclopedias.

He said those books describe him as a country singer, balladeer, composer of "Okie from Muskogee" and "the poet of the common man.

On December 5, , Haggard was treated at an undisclosed hospital in California for pneumonia. In March , Haggard was once again hospitalized. Kennedy Center for the Performing Arts in recognition of his lifetime achievement and "outstanding contribution to American culture.

It stretches from North Chester Avenue west to U. Route 99 and provides access to the William M. Thomas airport terminal at Meadows Field Airport.

Haggard played two shows to raise money to pay for the changes in road signage. Haggard stepped to the podium and said, "Thank you.

Although he was outspoken in his dislike for modern country music, [80] he praised George Strait , Toby Keith , and Alan Jackson.

Keith has singled out Haggard as a major influence on his career. Merle appeared in season five, episode three of The Waltons called " The Comeback ".

He played Red Turner, a local musician who had become depressed and withdrawn after the death of his son, played by Ron Howard , in the episode called " The Gift ".

Academy of Country Music []. Country Music Hall of Fame and Museum. Nashville Songwriters Hall of Fame. Oklahoma Music Hall of Fame. From Wikipedia, the free encyclopedia.

Country outlaw country Bakersfield sound. Merle was a real westerner. Like one of those lizards that thrives in arid heat.

He was a California guy, but not the California you see on television with Palm Trees. Retrieved April 7, Retrieved September 30, Archived from the original on July 27, Retrieved April 9, Country Music Hall of Fame.

Archived from the original on March 31, Retrieved April 16, Archived from the original on April 19, The New York Times. Archived from the original on June 26, Retrieved June 2, Retrieved April 5, Retrieved June 19, Retrieved April 6, The Life and Times of a Badass Legend".

Down Every Road — Liner notes. Queen of the Coast Liner notes. Archived from the original on February 7, Learning to Live With Myself".

The History of American Country. A , 2nd rev. University of Texas Press, Austin, , p. American Culture in the s. Hot Country Songs page 1 ".

Archived from the original on April 8, Retrieved April 8, Archived from the original on January 3, Retrieved January 28, Retrieved October 19, Archived from the original on June 27, Retrieved December 26, Retrieved 14 April Retrieved July 22, Archived from the original on April 2, Explicit use of et al.

Retrieved July 3, Archived from the original on January 23, Retrieved November 19, Archived from the original on September 14, Betts December 6, Retrieved December 6, Retrieved March 20, Archived from the original on April 6, Archived from the original on April 7, Retrieved April 13, Retrieved October 28, Emotional Haggard Accompanies Boxcar to Museum".

Now It Needs Work". Archived from the original on April 22, All Music Guide to the Blues 3rd ed. Narrative, Cognition, and Country-and-Western Music".

The Grateful Dead Family Discography. Archived from the original on March 28, Lynyrd Skynyrd and White Southern Manhood".

White Masculinity in the Recent South. Louisiana State University Press. Hot Country Songs page 2 ". Hot Country Songs page 3 ". Hot Country Songs page 4 ".

Academy of Country Music. The First 11 To Be Continued The Epic Years Hag: The Best of Merle Haggard. Unilateral thyroidectomy was performed in a mean of 54 minutes with excellent aesthetic results.

The new device is a promising feature to allow safe transoral thyroid surgery in a cadaver model. Further studies in clinical series are needed to evaluate the broad application of the device.

Nowadays, minimally invasive thyroid and parathyroid gland resections for both benign and malignant tumors are rarely performed.

Recently, promising new endoscopic transoral approaches to the anterior neck have been described with good results and few complications. This study describes the first clinical series in Germany using transoral endoscopic thyroidectomy-vestibular approach TOETVA and identifies technical issues and solutions.

The technique is indicated for hemithyroidectomy in patients without pre-existing neck operations. The technical steps consist of a 10 mm incision at the center of the oral vestibule, followed by subplatysmal hydrodissection.

A blunt dissector stick is inserted creating a space below the platysma to the anterior neck and the infrahyoid muscles then three trocars are inserted in the vestibular area.

After separation of the infrahyoid muscles, the thyroid isthmus is transected. Anatomical structures, such as the superior thyroid artery, parathyroid glands and the recurrent laryngeal nerve can be easily identified with magnification.

Intraoperative neuromonitoring is used routinely, adding safety in avoiding nerve damage. An optimal operative field due to subplatysmal dissection enables exposure of the thyroid and parathyroid glands.

Several critical steps and suitable solutions were identified in the study. Lateral and upper positioning of lateral trocars avoiding mental nerve injury.

Initial hydrodissection of the subplatysmal space. Use of one dissector progressively creating the operative space in the anterior cervical region.

Using internal-external sutures to retract the infrahyoid muscles. Intraoperative neuromonitoring used routinely through the trocars or percutaneously through a 1 mm incision.

Extraction of the specimen through a recovery bag. Drainages are possible, but can be avoided in small operative fields. The new TOETVA technique for thyroid surgery is a promising option for selected patients to enable transoral thyroid and parathyroid surgery through the vestibular approach.

Further studies in clinical series, especially regarding safety are needed to evaluate the indications of the technique.

Immunosuppression following liver transplantation and the course of inflammatory bowel disease - A case control study. Aim The aim of this study was to investigate the influence of immunosuppression following orthotopic liver transplantation OLT on course of inflammatory bowel disease IBD including disease activity and complications.

All drugs including immunosuppressive and anti-inflammatory medication and complications during follow-up were recorded in 6-month intervals.

Also, surgical interventions before and after OLT as well as endoscopic interventions with macroscopic and microscopic findings were collected and analyzed.

Additionally, development of malignant neoplasias was recorded. The mean follow-up was 7. Short-term therapy with calcineurin inhibitors or mycophenolate mofetil led to short-term remission, yet sustained remission could only be achieved in patients receiving mycophenolate mofetil.

Patients in the control group required nearly 2 times as many surgical interventions related to IBD than patients in the transplant group.

Conclusions Our data demonstrate an overall positive impact of immunosuppression following OLT on the course of IBD, especially with mycophenolate mofetil, but an increased rate of malignancies.

Transoral endoscopic thyroid surgery: Aug Viszeralmedizin The new era of transoral endoscopic thyroid surgery: Livers discarded after standard organ retrieval are commonly used as a cell source for hepatocyte transplantation.

Due to the scarcity of organ donors, this leads to a shortage of suitable cells for transplantation. Here, the isolation of liver cells from diseased livers removed during liver transplantation is studied and compared to the isolation of cells from liver specimens obtained during partial liver resection.

There were no differences in viability, cell integrity and metabolic activity in cell culture and survival after cold storage when comparing the hepatocytes from the Rx-group and the Ex-group.

In some cases, the latter group showed tendencies of increased resistance to isolation and storage procedures. Progenitor cell enrichment was not sufficient for clinical application.

Hepatocytes isolated from diseased explanted livers showed the essential characteristics of being adequate for cell transplantation.

Increased numbers of liver progenitor cells can be isolated from diseased explanted livers. These results support the feasibility of using diseased explanted livers as a cell source for liver cell transplantation.

Proof of Concept for in vivo Tissue Engineering. Stable long-term functioning of liver cells after transplantation in humans is still not achieved successfully.

A new approach for successful engraftment of liver cells may be the transplantation of syngeneic cells into an allogeneic liver graft. We therefore developed a new rat model for combined liver and liver cell transplantation cLCTx under stable immunosuppression.

After inducing a mitotic block, liver grafts from female donor rats Dark Agouti were transplanted into female recipients Lewis. In male Lewis rats, liver cell proliferation was induced with subsequent cell isolation and transplantation into female recipients after organ transplantation.

Y-chromosome detection of the transplanted male cells was performed by quantitative polymerase chain reaction qPCR and fluorescence in situ hybridization FisH with localization of transplanted cells by immunohistochemistry.

Immunohistochemistry demonstrated the engraftment of transplanted cells, as confirmed by FisH, showing repopulation of the liver graft with Engraftment of transplanted syngeneic cells after cLCTx was achieved for up to 90 days under immunosuppression.

Immunohistochemistry indicated cell proliferation, and the FisH results were partly confirmed by qPCR. This new protocol in rats appears feasible for addressing long-term functioning and eventually the induction of operational tolerance in the future.

Patients who have a liver transplant for primary sclerosing cholangitis may develop recurrent disease and biliary complications, organ loss necessitating revision liver transplant, or death.

We evaluated long-term outcomes in patients who had liver transplant for primary sclerosing cholangitis. In 71 patients who had a liver transplant for end-stage liver disease because of primary sclerosing cholangitis, a retrospective review was done to evaluate biliary complication-free survival, transplanted organ survival, and death.

Human leukocyte antigen typing and matching were reviewed. The 5- and year event-free survival reached Male sex of transplant recipients was a significant risk factor for biliary complications, revision liver transplant, or death.

Most patients had inflammatory bowel disease, primarily ulcerative colitis. The human leukocyte antigen profile or number of mismatches had no effect on complication-free survival.

Biliary complications, revision liver transplant, and death are a useful combined primary endpoint for recurrent primary sclerosing cholangitis after liver transplant.

Cellular therapies require methods for non-invasive visualization of transplanted cells. Micron-sized iron oxide particles MPIOs generate strong contrast in Magnetic Resonance Imaging MRI and are therefore ideally suited as an intracellular contrast agent to image cells under clinical conditions.

However, MPIOs were previously not applicable for clinical use. Here, we present the development and evaluation of silica-based micron-sized iron oxide particles sMPIOs with a functionalizable particle surface.

The particle surfaces were covered with COOH-groups. Monodisperse sMPIOs had an average size of 1. Particle uptake, toxicity, and imaging studies were performed using HuH7 cells and human and rat hepatocytes.

Labeling was stable and had no adverse effects on labeled cells. Silica is a biocompatible material that has been approved for clinical use. Moreover, the particle surface provides the opportunity to create multifunctional particles for targeted delivery and diagnostics.

Safety and efficacy of new integrated bipolar and ultrasonic scissors compared to conventional laparoscopic 5-mm sealing and cutting instrument.

Hemostasis is a central issue in laparoscopic surgery. Ultrasonic scissors and bipolar clamps are commonly used, with known advantages with each technique.

As safety parameters, temperature profiles after single activation and after a defined cut were determined. As efficacy parameters, seal failures and the maximum burst pressure BP were measured after in vivo sealing of vessels of various types and diameters categories and mm.

Moreover, the vertical width of the tissue seal was measured on serial histological slices of selected arteries. The cutting speed was measured during division of isolated arteries and during dissection of a defined length of compound tissue 10 cm of mesentery.

Burst-pressure measurement and histological analysis were performed by investigators blinded to the used sealing device. No differences in the rate of seal failures were observed.

The cutting speed of the TB was significantly higher than that of all other devices. The maximum temperature of the HA and the TB was significantly higher.

No relevant differences were observed between the HA and the TB. The ultrasonic and bipolar technique of the TB has the potential to surpass the dissection speed of ultrasonic devices with the sealing efficacy of bipolar clamps.

However, heat production that is comparable to conventional ultrasonic scissors should be minded for clinical use.

An operational concept for long-term cinemicrography of cells in mono- and co-culture under highly controlled conditions - The SlideObserver.

Cell morphology, proliferation and motility, as well as mono- and heterotypic cell-to-cell interactions, are of increasing interest for in vitro experiments.

However, tightly controlling culture conditions whilst simultaneously monitoring the same set of cells is complicated.

Chris Guillebeau wie kaufe ich ethereum of: Es war ein Hin und Her. When he returned wie alt ist michael ballack same year, he and his friend were arrested for robbery. Labeling beliebte lottozahlen stable and had no adverse effects on labeled cells. Moreover, the particle surface provides the opportunity to create multifunctional particles for targeted delivery and diagnostics. Aug Viszeralmedizin The new era of transoral endoscopic thyroid surgery: The new device is a promising feature to allow safe transoral thyroid surgery in a cadaver model. American Culture in the s. Archived from the clams casino rainforest ep free download on April 19, He followed it in with Roots, vol. I could tell he was in a dark mood Haggard endorsed Fender guitars and had a Custom Artist signature model Telecaster. He shared the writing credit with Owens for his hit Today I Started Loving You Martina mörl and acknowledged, including on stage, that the song was about a sudden burst of special feelings he experienced for her while they were touring together. Nowadays, minimally invasive thyroid and parathyroid gland resections for both benign and malignant tumors are rarely wm fussball ergebnisse. Particle uptake, toxicity, and imaging studies were performed using HuH7 cells and human and rat hepatocytes.

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Ihr wurde die Gebärmutter mit 43 Jahren weggenommen. Nach seinem Dschungel-Auszug muss Yotta ganz offensichtlich erstmal Dampf ablassen. Sie wurden immer mehr und ich bekam auf einmal Blutungen. Der Sänger selbst bestätigte die traurige Nachricht selbst im "Bunte"-Interview. In den letzten fünf Jahren haben wir sehr um unsere Ehe gekämpft. Für Kritik oder Anregungen füllen Sie bitte die nachfolgenden Felder aus. Die Krankheit war früher als Selbstmordkrankheit bekannt, weil sich die Leute umbrachten, wenn sie die Schmerzen nicht mehr ertragen konnten" , verriet Markus Mörl schon vor zwei Jahren.

He also scored a number-one hit in with " Bar Room Buddies ," a duet with actor Clint Eastwood that appeared on the Bronco Billy soundtrack.

Haggard appeared in an episode of The Waltons entitled "The Comeback," season five, episode three, original air-date October 10, He played a band leader named Red, who had been depressed since the death of his son Ron Howard.

The same year, he alternately spoke and sang the ballad "The Man in the Mask. Haggard also changed record labels again in , moving to Epic and releasing one of his most critically acclaimed albums, Big City , on which he was backed by The Strangers.

Nelson believed the Academy Award -winning film Tender Mercies , about the life of fictional singer Mac Sledge, was based on the life of Merle Haggard.

Actor Robert Duvall and other filmmakers denied this and claimed the character was based on nobody in particular.

In , Haggard and his third wife Leona Williams divorced after five stormy years of marriage. The split served as a license to party for Haggard, who spent much of the next decade becoming mired in alcohol and drug problems.

He said in an interview from this period: Why do I like this now? Haggard was hampered by financial woes well into the s, as his presence on the charts diminished in favor of newer country singers, such as George Strait and Randy Travis.

After CBS Records Nashville avoided releasing the song, Haggard bought his way out of the contract and signed with Curb Records , which was willing to release the song.

In , Haggard made a comeback of sorts, signing with the independent record label Anti and releasing the spare If I Could Only Fly to critical acclaim.

He followed it in with Roots, vol. In December , Haggard spoke at length on Larry King Live about his incarceration as a young man and said it was "hell" and "the scariest experience of my life.

It was like a verbal witch-hunt and lynching. In October , Haggard released his album Chicago Wind to mostly positive reviews.

In , Haggard was going to perform at Riverfest in Little Rock, Arkansas , but the concert was canceled because he was ailing, and three other concerts were canceled, as well.

However, he was back on the road in June and successfully completed a tour that ended on October 19, Haggard collaborated with many other artists over the course of his career.

Merle arrived on the second day, devastated that he would not get to record with him, but the album helped return Wills to public consciousness, and set off a Western swing revival.

In he appeared on For the Last Time: Bob Wills and His Texas Playboys. Merle was very nice, very sweet, but he had his own enemies and his own demons.

In , Haggard dispelled the notion that Parsons was any kind of outlaw, telling Mark Rose, "He was a pussy.

Hell, he was just a long-haired kid. I thought he was a good writer. He was not wild, though. All these guys running around in long hair talking about being wild and Rolling Stones.

It might determine how ignorant they are. In , a second, less successful LP, Seashores of Old Mexico , was also released, and the pair would work together again with Ray Price in , releasing the album Last of the Breed.

The two also recorded another duet album Django and Jimmie. The album, on which they were backed by The Strangers , was not a hit, peaking at number In , Haggard released an album of gospel songs with Albert E.

Brumley called Two Old Friends. Haggard endorsed Fender guitars and had a Custom Artist signature model Telecaster.

The guitar is a modified Telecaster Thinline with laminated top of figured maple, set neck with deep carved heel, birdseye maple fingerboard with 22 jumbo frets, ivoroid pickguard and binding, gold hardware, abalone Tuff Dog Tele peghead inlay, 2-Colour Sunburst finish, and a pair of Fender Texas Special Tele single-coil pickups with custom-wired 4-way pickup switching.

He also played six-string acoustic models. Haggard was married five times, first to Leona Hobbs from to They had four children: Dana, Marty , Kelli, and Noel.

Shortly after divorcing Hobbs, in , he married singer Bonnie Owens. He shared the writing credit with Owens for his hit Today I Started Loving You Again and acknowledged, including on stage, that the song was about a sudden burst of special feelings he experienced for her while they were touring together.

Haggard and Owens divorced in , but remained close friends as Owens continued as his backing vocalist until her death in In , Haggard married Leona Williams.

In , they divorced. They had two children, Jenessa and Ben. Haggard said he started smoking marijuana when he was 41 years old.

Haggard underwent angioplasty in to unblock clogged arteries. He described himself as a student of music, philosophy and communication. He would discuss jazzman Howard Roberts guitar playing, life after death and the unique speaking technique of Garner Ted Armstrong of The World Tomorrow with enthusiasm and authority.

He was like a professor to me. What education I have, I owe to him. He is listed in the Guinness Book of World Records and many encyclopedias.

He said those books describe him as a country singer, balladeer, composer of "Okie from Muskogee" and "the poet of the common man.

On December 5, , Haggard was treated at an undisclosed hospital in California for pneumonia. In March , Haggard was once again hospitalized. Kennedy Center for the Performing Arts in recognition of his lifetime achievement and "outstanding contribution to American culture.

It stretches from North Chester Avenue west to U. Route 99 and provides access to the William M. Thomas airport terminal at Meadows Field Airport.

Haggard played two shows to raise money to pay for the changes in road signage. Haggard stepped to the podium and said, "Thank you. Although he was outspoken in his dislike for modern country music, [80] he praised George Strait , Toby Keith , and Alan Jackson.

Keith has singled out Haggard as a major influence on his career. Merle appeared in season five, episode three of The Waltons called " The Comeback ".

He played Red Turner, a local musician who had become depressed and withdrawn after the death of his son, played by Ron Howard , in the episode called " The Gift ".

Academy of Country Music []. Country Music Hall of Fame and Museum. Nashville Songwriters Hall of Fame. Oklahoma Music Hall of Fame. From Wikipedia, the free encyclopedia.

Country outlaw country Bakersfield sound. Merle was a real westerner. Like one of those lizards that thrives in arid heat. He was a California guy, but not the California you see on television with Palm Trees.

Retrieved April 7, Retrieved September 30, Archived from the original on July 27, Retrieved April 9, Country Music Hall of Fame.

Archived from the original on March 31, Retrieved April 16, Archived from the original on April 19, The New York Times. Archived from the original on June 26, Retrieved June 2, Retrieved April 5, Retrieved June 19, Retrieved April 6, The Life and Times of a Badass Legend".

Down Every Road — Liner notes. Queen of the Coast Liner notes. Archived from the original on February 7, Learning to Live With Myself".

The History of American Country. A , 2nd rev. University of Texas Press, Austin, , p. American Culture in the s. Hot Country Songs page 1 ".

Archived from the original on April 8, Retrieved April 8, Archived from the original on January 3, Retrieved January 28, Retrieved October 19, Archived from the original on June 27, Retrieved December 26, Transoral endoscopic thyroid surgery: Aug Viszeralmedizin The new era of transoral endoscopic thyroid surgery: Livers discarded after standard organ retrieval are commonly used as a cell source for hepatocyte transplantation.

Due to the scarcity of organ donors, this leads to a shortage of suitable cells for transplantation. Here, the isolation of liver cells from diseased livers removed during liver transplantation is studied and compared to the isolation of cells from liver specimens obtained during partial liver resection.

There were no differences in viability, cell integrity and metabolic activity in cell culture and survival after cold storage when comparing the hepatocytes from the Rx-group and the Ex-group.

In some cases, the latter group showed tendencies of increased resistance to isolation and storage procedures. Progenitor cell enrichment was not sufficient for clinical application.

Hepatocytes isolated from diseased explanted livers showed the essential characteristics of being adequate for cell transplantation. Increased numbers of liver progenitor cells can be isolated from diseased explanted livers.

These results support the feasibility of using diseased explanted livers as a cell source for liver cell transplantation.

Proof of Concept for in vivo Tissue Engineering. Stable long-term functioning of liver cells after transplantation in humans is still not achieved successfully.

A new approach for successful engraftment of liver cells may be the transplantation of syngeneic cells into an allogeneic liver graft.

We therefore developed a new rat model for combined liver and liver cell transplantation cLCTx under stable immunosuppression.

After inducing a mitotic block, liver grafts from female donor rats Dark Agouti were transplanted into female recipients Lewis.

In male Lewis rats, liver cell proliferation was induced with subsequent cell isolation and transplantation into female recipients after organ transplantation.

Y-chromosome detection of the transplanted male cells was performed by quantitative polymerase chain reaction qPCR and fluorescence in situ hybridization FisH with localization of transplanted cells by immunohistochemistry.

Immunohistochemistry demonstrated the engraftment of transplanted cells, as confirmed by FisH, showing repopulation of the liver graft with Engraftment of transplanted syngeneic cells after cLCTx was achieved for up to 90 days under immunosuppression.

Immunohistochemistry indicated cell proliferation, and the FisH results were partly confirmed by qPCR. This new protocol in rats appears feasible for addressing long-term functioning and eventually the induction of operational tolerance in the future.

Patients who have a liver transplant for primary sclerosing cholangitis may develop recurrent disease and biliary complications, organ loss necessitating revision liver transplant, or death.

We evaluated long-term outcomes in patients who had liver transplant for primary sclerosing cholangitis. In 71 patients who had a liver transplant for end-stage liver disease because of primary sclerosing cholangitis, a retrospective review was done to evaluate biliary complication-free survival, transplanted organ survival, and death.

Human leukocyte antigen typing and matching were reviewed. The 5- and year event-free survival reached Male sex of transplant recipients was a significant risk factor for biliary complications, revision liver transplant, or death.

Most patients had inflammatory bowel disease, primarily ulcerative colitis. The human leukocyte antigen profile or number of mismatches had no effect on complication-free survival.

Biliary complications, revision liver transplant, and death are a useful combined primary endpoint for recurrent primary sclerosing cholangitis after liver transplant.

Cellular therapies require methods for non-invasive visualization of transplanted cells. Micron-sized iron oxide particles MPIOs generate strong contrast in Magnetic Resonance Imaging MRI and are therefore ideally suited as an intracellular contrast agent to image cells under clinical conditions.

However, MPIOs were previously not applicable for clinical use. Here, we present the development and evaluation of silica-based micron-sized iron oxide particles sMPIOs with a functionalizable particle surface.

The particle surfaces were covered with COOH-groups. Monodisperse sMPIOs had an average size of 1. Particle uptake, toxicity, and imaging studies were performed using HuH7 cells and human and rat hepatocytes.

Labeling was stable and had no adverse effects on labeled cells. Silica is a biocompatible material that has been approved for clinical use.

Moreover, the particle surface provides the opportunity to create multifunctional particles for targeted delivery and diagnostics.

Safety and efficacy of new integrated bipolar and ultrasonic scissors compared to conventional laparoscopic 5-mm sealing and cutting instrument. Hemostasis is a central issue in laparoscopic surgery.

Ultrasonic scissors and bipolar clamps are commonly used, with known advantages with each technique. As safety parameters, temperature profiles after single activation and after a defined cut were determined.

As efficacy parameters, seal failures and the maximum burst pressure BP were measured after in vivo sealing of vessels of various types and diameters categories and mm.

Moreover, the vertical width of the tissue seal was measured on serial histological slices of selected arteries. The cutting speed was measured during division of isolated arteries and during dissection of a defined length of compound tissue 10 cm of mesentery.

Burst-pressure measurement and histological analysis were performed by investigators blinded to the used sealing device.

No differences in the rate of seal failures were observed. The cutting speed of the TB was significantly higher than that of all other devices.

The maximum temperature of the HA and the TB was significantly higher. No relevant differences were observed between the HA and the TB.

The ultrasonic and bipolar technique of the TB has the potential to surpass the dissection speed of ultrasonic devices with the sealing efficacy of bipolar clamps.

However, heat production that is comparable to conventional ultrasonic scissors should be minded for clinical use.

An operational concept for long-term cinemicrography of cells in mono- and co-culture under highly controlled conditions - The SlideObserver.

Cell morphology, proliferation and motility, as well as mono- and heterotypic cell-to-cell interactions, are of increasing interest for in vitro experiments.

However, tightly controlling culture conditions whilst simultaneously monitoring the same set of cells is complicated. Moreover, video-microscopy of distinct cells or areas of cells over a prolonged period of time represents a technical challenge.

The SlideObserver was designed for cinemicrography of cells in co-and monoculture. The core elements of the system are the SlideReactors, miniaturised hollow fibre-based bioreactors operated in closed perfusion loops.

Within the SlideReactors, cells can be cultured under adaptable conditions as well as in direct- and indirect co-culture. The independent perfusion loops enable controlled variation of parameters such as medium, pH, and oxygenation.

A combined automated microscope stage and camera set-up allows for micrograph acquisition of multiple user-defined regions of interest within the bioreactor units.

The operational system enabled non-stop imaging and automated control of process parameters as well as elective manipulation of either reactor.

As opposed to non-perfused culture systems or comparable devices for cinemicrographic analysis, the SlideObserver allows simultaneous morphological monitoring of an entire culture of cells in multiple bioreactors.

Despite the great potential of MPIOs for in vivo imaging of labeled cells, little is known on the intracellular localization of these particles following uptake due to the lack of techniques with the ability to monitor the particle uptake in vivo at single-cell level.

Here, we show that coherent anti-Stokes Raman scattering CARS microscopy enables non-invasive, label-free imaging of MPIOs in living cells with sub-micron resolution in three dimensions.

CARS allows simultaneous visualization of the cell framework and the MPIOs, where the particles can be readily distinguished from other cellular components of comparable dimensions, and localized inside the cell.

Labeling of hepatocytes with micron-sized iron oxide particles MPIOs enables cell detection using clinical magnetic resonance equipment.

For clinical applications, large numbers of cells must be labeled in a simple and rapid manner and have to be applied in suspension. However, all existing protocols are based on adhesion culture labeling with subsequent resuspension, only suitable for small experimental settings.

The aim of this study was to investigate the feasibility of preparing MPIO-labeled primary human hepatocytes in a temporary suspension culture.

Particle incorporation was investigated by light and electron microscopy. Cells were compared with adhesion culture-labeled and subsequently enzymatically resuspended cells.

Suspension cultures showed a higher outcome in cell recovery compared with the conventional labeling method.

Labeling in suspension had no adverse effects on cell integrity or metabolic activity. We conclude that labeling of human hepatocytes in suspension is feasible and simple and may serve future large-scale processing of cells.

Anastomotic leaks after oesophagojejunostomy usually are treated by endoluminal stenting with self-expandable metal or plastic stents.

Here we present a patient with more than 4 years of oesophageal stenting for anastomotic leakage after gastrectomy. During the attempted removal of the stent he experienced a perforation of the jejunum.

Emergency surgery with complete resection of the stent and transhiatal oesophagojejunostomy was performed.

Generally, early removal of oesophageal stents weeks after implantation is recommended, as later attempts often fail and may lead to extensive surgery.

Development and in vitro evaluation of Silica-based iron oxide particles suitable for clinical application of cellular imaging with MRI.

Magnetic Resonance Imaging MRI can be used for non-invasive monitoring after liver cell transplantation LCT , but clinical applicable micron-sized particles are not available.

Aim of this study was the development a new class of micron-sized particles for cellular imaging based on clinical applicable materials.

We developed silica-based particles 1. Huh7-cells, primary rat hepatocytes, and primary human hepatocytes were used for in vitro evaluation.

Phantom studies were performed using a 3. Skip to content 8.

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Martina Mörl Video

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